Akademik Kadro

Prof. Dr. Nevin Gül Karagüler

Akademik dereceler ve çalışma deneyimi

Prof. Dr. – İstanbul Teknik Üniversitesi, Fen-Edebiyat Fakültesi, Moleküler Biyoloji ve Genetik Bölümü , İstanbul, Turkey (Şubat 2017- Halen)

Doç. Dr. – İstanbul Teknik Üniversitesi, Fen-Edebiyat Fakültesi, Moleküler Biyoloji ve Genetik Bölümü , İstanbul, Turkey (Temmuz 2002-Şubat 2017)

Öğretim Görevlisi. – İstanbul Teknik Üniversitesi, Fen-Edebiyat Fakültesi, Moleküler Biyoloji ve Genetik Bölümü , İstanbul, Turkey (Ekim 2000 – Temmuz 2002)

Araştırma görevlisi – Celal Bayar Üniversitesi, Kimya Bölümü, İzmir (Mayıs 1999 – Ekim 2000)

Doktora – Bristol Üniversitesi, Biyokimya Bölümü, Bristol, UK (1999)

Yüksek Lisans – Bristol Üniversitesi, Biyokimya Bölümü, Bristol, UK (1995)

Lisans – Ege Üniversitesi, Fen Fakültesi, Kimya Bölümü, İzmir (1992)

İlgi alanları

Endüstriyel uygulaması olan enzimlerin (bsLDH, cmFDH) özelliklerinin protein mühendisliği teknikleri kullanılarak iyileştirilmesi

Inorganik yüzeye spesifik polypeptidlerin bağlanmadan sorumlu amino asitlerinin site directed mutagenesis tekniği kullanılarak tanımlanması

Inorganik yüzeye spesifik bakteriophage M13 bağlanma afinitesinin ve seçiciliğinin protein mühendisliği teknikleri kullanılarak gelistirilmesi

Yayınlar

A.1. Gul-Karaguler, N., R.B. Sessions, A.R. Clarke ve J.J. Holbrook, “A single mutation in the NAD-specific formate dehydrogenase from candida methylica allows the enzyme to use NADP” Biotechnology Letters, 23, 283-287 (2001).
A.2. Gul-Karaguler, N., R.B. Sessions ve J.J. Holbrook, “Role of glutamate-52 in the mechanism of L-lactate dehydrogenase from bacillus stearothermophilus” Biotechnology Letters, 23, 395-399 (2001).
A.3. Karaguler, N.G., R.B. Sessions, K.M. Moreton, A.R. Clarke ve J.J. Holbrook, “Estimating the energetic contribution of hydrogen bonding to the stability of C. methylica formate dehydrogenase by using double mutant cycles” Biotechnology Letters, 14, 1137-1140 (2004).
A.4. Karaguler, N.G., R.B. Sessions ve A.R. Clarke, “Effects of disulphide bridges onto stability of formate dehydrogenase from candida methylica,” Biotechnology Letters, 29, 1375-1380 (2007).
A.5. Ordu, E.B. ve N.G. Karaguler, “Improving the purification of NAD+-dependent formate dehydrogenase from candida methylica,” Preparative Biochemistry and Biotechnology, 37 (4), 333 – 341 (2007).
A.6. Karaguler, N.G., R.B. Sessions, B. Binay, E.B. Ordu ve A.R. Clarke “Protein Engineering Applications on Industrially Important Enzymes: bacillus stearothermophilus LDH and candida methylica FDH,” Biochemical Society Transactions, 35 (6), 1610-1615 (2007).
A.7. Binay,B. ve N.G. Karaguler, “Attempting to remove the substrate inhibition of L-lactate dehydrogenase from Bacillus stearothermophilus by site-directed mutagenesis,” Applied Biochemistry & Biotechnology, 141 (2-3), 265-272 (2007).
A.8. Kacar, T., Zin, MT., So, C., Wilson, B., Ma, H., Gul-Karaguler, N., Jen, A.K.Y., Sarikaya, M., Tamerler, C., “Directed Self-immobilization of alkaline phosphatase on micro-patterned substrates via genetically fused metal binding peptide,” Biotechnology and Bioengineering, 103 (4), 696-705 (2009).
A.9. Ordu, E.B., Cameron, G., Clarke, A.R. ve N.G. Karagüler “Kinetic and thermodynamic properties of the folding and assembly of formate dehydrogenase” FEBS Letters 583 (17), 2887–2892 (2009).
A.10. Binay, B., Shoemark, D.K., Sessions, R.B. Clarke, A.R. ve N.G. Karaguler “Increasing the substrate specificity of Bacillus stearothermophillus lactate dehydrogenase by DNA shuffling” Biochemical Engineering Journal 48, 118–123 (2009)
A.11. Oktay, HI; Heperkan, D; Yelboga, E; Karaguler, NG (2011) Aspergillus flavus – primary causative agent of aflatoxins in dried figs, Mycotaxon, Vol: 115 Pages: 425-433
A.12. Balci, N; Vardar, N; Yelboga, E; Karaguler, NG (2012) Bacterial community composition of sediments from Artificial Lake Maslak, Istanbul, Turkey, Environmental Monitoring and Assessment, Vol: 184, Pages: 5641-5650
A.13. Cetinel, S; Dincer, S; Cebeci, A; Oren, EE; Whitaker, JD; Schwartz, DT; Karaguler, NG; Sarıkaya, M; Tamerler, C (2012) Peptides to bridge biological-platinum materials interface, Bioinspired, Biomimetic and Nanobiomaterials, Vol:1, Pages:143-153
A.14. Kahraman, M; Ordu, EB; Yesiladali, K; Karaguler, NG; Tamerler, C (2012) Improved Candida Methylica Formate Dehydrogenase Fermentation Through Statistical Optimization Of Low-Cost Culture Media, Preparatıve Biochemistry & Biotechnology Vol: 42: 507-519
A.15. Ordu, EB; Yelboga, E; Secundo, F; Sessions, RB; Karaguler, NG (2012) The effect of methionine to cysteine substitution on the stability of formate dehydrogenase from Candida methylica, Journal of Molecular Catalysis B-Enzymatic, Vol: 82 Pages: 109-114
A.16. Sibel Cetinel, H. Burak Caliskan, Deniz T. Yucesoy, A. Senem Donatan, Esra Yuca, Mustafa Urgen, Nevin G. Karaguler and Candan Tamerler (February 2013) Addressable self-immobilization of lactate dehydrogenase across multiple length scales, Biotechnology Journal, Vol:8, Issue;2 Pages:262-272
A.17. Binay, B., Sessions RB., Karaguler, NG (2013) “A double mutant of highly purified Geobacillus stearothermophilus lactate dehydrogenase recognises L-mandelic acid as a substrate”, Enzyme and Microbial Technology, Vol: 52, Issue:6-7 Pages: 393-399 (B)
A.18. Ordu, EB., Sessions, RB., Clarke, AR., Karaguler, NG. (2013) “Effect of surface electrostatic interactions on the stability and folding of formate dehydrogenase from Candida methylica” Journal of Molecular Catalysis B-Enzymatic, Vol: 95 Pages: 23-28 (B)
A.19. Yücesoy, D.T., Karaca, B.T., Cetinel, S., Çalışkan, H.B., Adali, E., Karagüler, N.G. (2014) “Direct Bioelectroanalysis at the interfaces by genetically engineered dehydrogenase” Bioinspired, Biomimetic and Nanobiomaterials Vol: 4, Issue: 1 Pages: 79-89
A.20. Teker, D., Muhaffel, F., Menekse, M., Karaguler, NG., Baydogan, M., Cimenoglu, H.(2015) “Characteristics of multi-layer coating formed on commercially pure titanium for biomedical applications” Materials Science & Engineering C-Materials for Biological Applications Vol: 48 Pages: 579-585 (B)
A.21. Özgün, G., Karagüler, NG., Turunen, O., Turner, NJ., Binay, B. (2015) “Characterization of a new acidic NAD+-dependent formate dehydrogenase from thermophilic fungus Chatemium thermophilum” Journal of Molecular Catalysis B: Enzymatic Vol: 122 Pages: 212-217 (B)
A.22. Balcı, N., Menekşe, M., Karagüler, NG., Sönmez ŞM., Meister, P. (2015) “Reproducing authigenic carbonate precipitation in the hypersaline Lake Acıgöl (Turkey) with microbial cultures” Geomicrobiology Journal (B) Vol: 33 Issue: 9 Pages: 758-773.
A.23. Sevinç Aşkın Aslan, William Birmingham, Nevin Gül Karagüler, Nicholas J. Turner, Barış Binay (2016) “Semi-rational design of Bacillus stearothermophilus lactate dehydrogenase to access various chiral α-hydroxy acids” Applied Biochemistry and Biotechnology (C) Vol: 179 Issue: 3 Pages: 474-484. ©.
A.24. Faiz Muhaffel, Grzegorz Cempura, Meryem Menekşe, Aleksandra Czyrska-Filemonowicz, Nevin Karagüler, Hüseyin Çimenoğlu (2016) “Characteristics of multi-layer coatings synthesized on Ti6Al4V alloy by micro-arc oxidation in silver nitrate added electrolytes” Surface & Coatings Technology Vol:307, Pages:308-315 (A)
A.25. Ozgun, Gulsah P.; Ordu, Emel B.; Tutuncu, H. Esra; Yelboga, Emrah, Sessions, Richard B.; Karaguler, Nevin Gul (2016)“Site Saturation Mutagenesis Applications on Candida methylica Formate Dehydrogenase” Scientifica DOI: 10.1155/2016/4902450

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